Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
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Date
2024-03-12Author
Schmitt, Luisa O.
Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
ORCID
https://orcid.org/0000-0002-5127-5640https://orcid.org/0000-0001-9032-1411
https://orcid.org/0000-0003-3151-4234
Metadata
Show full item recordAbstract
Consumption of high-fat diets (HFD) is associated with brain alterations, including changes
in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation
and brain dysfunction; however, this process is not entirely understood. We hypothesized
that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function.
The C6 (astrocytes) cell line was treated with palmitate or stearate (200 μM and 400 μM) for 6 h.
Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess
mitochondrial function, various parameters were measured (membrane potential, mass, respiration,
and complex activities). We observed that 6 h of treatment with 400 μM palmitate decreased
cell viability, and treatment with 200 μM palmitate changed the astrocyte morphology. Palmitate
increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate
significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex
I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial
respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and
impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular
morphology. Our study provides valuable insights into the potential mechanisms underlying the
relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related
brain dysfunction.
xmlui.dri2xhtml.METS-1.0.item-citation
Schmitt, L.O.; Blanco, A.; Lima, S.V.; Mancini, G.; Mendes, N.F.; Latini, A.; Gaspar, J.M. Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function. Metabolites 2024, 14, 161. https:// doi.org/10.3390/metabo14030161
Other links
https://www.mdpi.com/2218-1989/14/3/161https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/
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