Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
Date
2024-01-11Author
Herrera Lopez, Malena
Bertone Arolfo, Matías
Remedi, Mónica
Gastaldi, Laura
Wilson, Carlos
Guendulain, Gonzalo G.
Ceschin, Danilo
Cardozo Gizzi, Andrés
Cáceres, Alfredo
Moyano, Ana Lis
ORCID
https://orcid.org/0009-0008-2413-0340https://orcid.org/0009-0004-0826-119X
https://orcid.org/0000-0001-9719-331X
https://orcid.org/0000-0002-7497-5455
https://orcid.org/0000-0002-2583-9289
https://orcid.org/0000-0003-3894-1845
https://orcid.org/0000-0002-4163-9068
https://orcid.org/0000-0002-1089-8542
Metadata
Show full item recordAbstract
Extracellular vesicles (EVs) play a critical role in the development of neural cells in the central nervous system (CNS). Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. Here we show that hiPSCs and hNRs secrete EVs (hiPSC-EVs and hNR-EVs) with distinct protein cargoes. Remarkably, hNR-EVs carry neuronal and glial cellular components involved in CNS development. By in silico analysis, we found hNR-EVs protein signature is expressed in vivo and in vitro during human brain development. Importantly, hNR-EVs stimulate hiPSCs to change their cellular morphology with a significant reduction in the pluripotency regulator SOX2. Interestingly, these effects were inhibited by antibodies against an unexpected neuroglial cargo of hNR-EVs: the major proteolipid protein (PLP1). These findings show that hNRs secrete bioactive EVs containing neural components and might contribute as trophic factors during human CNS development.
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Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components
Malena Herrera Lopez, Matías Bertone Arolfo, Mónica Remedi, Laura Gastaldi, Carlos Wilson, Gonzalo G. Guendulain, Danilo Ceschin, Andrés Cardozo Gizzi, Alfredo Cáceres, Ana Lis Moyano
bioRxiv 2024.01.10.574187; doi: https://doi.org/10.1101/2024.01.10.574187
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