The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells
![Thumbnail](/bitstream/handle/11086/16760/journal.ppat.1008761.pdf.jpg?sequence=6&isAllowed=y)
View/ Open
Date
2020-08-13Author
Reinoso Vizcaíno, Nicolás Martín
Cian, Melina Beatriz
Cortez, Paulo R.
Olivero, Nadia B.
Hernández-Morfa, Mirelys
Piñas, Germán
Badapanda, Chandan
Rathore, Ankita
Perez, Daniel R.
Echenique, José Ricardo
Metadata
Show full item recordAbstract
The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus
pneumoniae following infection with epidemic or pandemic influenza A virus (IAV)
is well documented. However, the molecular mechanisms behind such synergism remain
largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with
S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal
two-component system SirRH appears essential for such enhanced survival. Through comparative
transcriptomic analysis between the ΔsirR and wt strains, a list of 179 differentially
expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB
Mn+2 transporter gene, which are involved in the stress response, are important in enhancing
S. pneumoniae survival in influenza-infected cells. The ΔsirR, ΔclpL and ΔpsaB deletion
mutants display increased susceptibility to acidic and oxidative stress and no enhancement
of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the
SirRH two-component system senses IAV-induced stress conditions and controls adaptive
responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.
xmlui.dri2xhtml.METS-1.0.item-citation
Reinoso-Vizcaíno NM, Cian MB, Cortes
PR, Olivero NB, Hernandez-Morfa M, Piñas GE, et
al. (2020) The pneumococcal two-component
system SirRH is linked to enhanced intracellular
survival of Streptococcus pneumoniae in influenzainfected pulmonary cells. PLoS Pathog 16(8):
e1008761. https://doi.org/10.1371/journal.
ppat.1008761
Collections
The following license files are associated with this item: