Artículos científicos - Año 2013http://hdl.handle.net/11086/258512024-03-28T08:39:48Z2024-03-28T08:39:48ZMechanical performance of encapsulated restorative glass-ionomer cements for use with Atraumatic Restorative Treatment (ART)Molina, Gustavo FabiánCabral, Ricardo JuanMazzola, IgnacioBrain Lascano, LauraFrencken, Jo. E.http://hdl.handle.net/11086/134912024-02-09T13:08:18Z2013-01-01T00:00:00ZMechanical performance of encapsulated restorative glass-ionomer cements for use with Atraumatic Restorative Treatment (ART)
Molina, Gustavo Fabián; Cabral, Ricardo Juan; Mazzola, Ignacio; Brain Lascano, Laura; Frencken, Jo. E.
The Atraumatic Restorative Treatment (ART) approach was suggested to be a suitable method to treat enamel and dentine carious lesions in patients with disabilities. The use of a restorative glass-ionomer with optimal mechanical properties is, therefore, very important. Objective: To test the null-hypotheses that no difference in diametral tensile, compressive and flexural strengths exists between: (1) The EQUIA system and (2) The Chemfil Rock (encapsulated glass-ionomers; test materials) and the Fuji 9 Gold Label and the Ketac Molar Easymix (hand-mixed conventional glass-ionomers; control materials); (3) The EQUIA system and Chemfil Rock. Material and Methods: Specimens for testing flexural (n=240) and diametral tensile (n=80) strengths were prepared according to standardized specifications; the compressive strength (n=80) was measured using a tooth-model of a class II ART restoration. ANOVA and Tukey B tests were used to test for significant differences between dependent and independent variables. Results: The EQUIA system and Chemfil Rock had significantly higher mean scores for all the three strength variables than the Fuji 9 Gold Label and Ketac Molar Easymix (α=0.05). The EQUIA system had significant higher mean scores for diametral tensile and flexural strengths than the Chemfil Rock (α=0.05).
Conclusion: The two encapsulated high-viscosity glass-ionomers had significantly higher test values for diametral tensile, flexural and compressive strengths than the commonly used hand-mixed high-viscosity glass-ionomers.
2013-01-01T00:00:00ZHuman Papillomavirus (HPV) in Oral Cavity Lesions: Comparison with Other Oral Cancer Risk FactorsVenezuela, Raúl FernandoTalavela, ÁngelFrutos, María CeciliaKeguen, Ana XimenaMonetti, Marina SoledadSollazo, MaríaPanico, RenéFerreyra de Prato, RuthCuffini, Cecilia Gabrielahttp://hdl.handle.net/11086/134882024-02-09T13:20:31Z2013-01-01T00:00:00ZHuman Papillomavirus (HPV) in Oral Cavity Lesions: Comparison with Other Oral Cancer Risk Factors
Venezuela, Raúl Fernando; Talavela, Ángel; Frutos, María Cecilia; Keguen, Ana Ximena; Monetti, Marina Soledad; Sollazo, María; Panico, René; Ferreyra de Prato, Ruth; Cuffini, Cecilia Gabriela
Human papillomavirus (HPV) is considered a necessary factor for the development of cervical cancer; however, its relationship with oral cancer is controversial. The aim of this study was detect the presence of HPV in lesions of the oral cavity and its correlation with other risk factors. Presence of HPV was studied by polymerase chain reaction in samples from benign lesions, potentially malignant lesions (PML), neoplasias and healthy mucosae. The results from the different groups were compared; in addition to their histopathological variables with tobacco smoking, alcohol consumption, so on. HPV was detected in 88.89% of benign lesions, 41.38% of PML and 56.25% of neoplasias. The most prevalent genotypes were 16 and 6. Together, reached 55% of the total number of cases. A significant association was observed between HPV and male gender, tobacco smokers, alcohol drinkers and benign lesions. Tobacco smoking and alcohol intake were associated to neoplasias. Our results showed that factors like tobacco smoking and alcohol drinking, have more influence than HPV in the development of oral neoplasias; however, 56.2% of the neoplasias tested positive for HPV; the percentage of HR-HPV detection increased with the severity of the lesions, suggesting its possible involvement in malignant processes
2013-01-01T00:00:00ZMalignancy Risk Models for Oral LesionsZarate, Ana MaríaBrezzo, María MagdalenaSecchi, Dante GustavoBarra, José LuisBrunotto Mabelhttp://hdl.handle.net/11086/134902024-02-05T15:59:19Z2013-01-01T00:00:00ZMalignancy Risk Models for Oral Lesions; Modelos de riesgo de malignidad para las lesiones orales
Zarate, Ana María; Brezzo, María Magdalena; Secchi, Dante Gustavo; Barra, José Luis; Brunotto Mabel
Objectives: The aim of this work was to assess risk habits, clinical and cellular phenotypes and TP53 DNA changes in oral mucosa samples from patients with Oral Potentially Malignant Disorders (OPMD), in order to create models that enable genotypic and phenotypic patterns to be obtained that determine the risk of lesions becoming malignant. Study Design: Clinical phenotypes, family history of cancer and risk habits were collected in clinical histories. TP53 gene mutation and morphometric-morphological features were studied, and multivariate models were applied. Three groups were estabished: a) oral cancer (OC) group (n=10), b) OPMD group (n=10), and c) control group (n=8). Results: An average of 50% of patients with malignancy were found to have smoking and drinking habits. A high percentage of TP53 mutations were observed in OC (30%) and OPMD (average 20%) lesions (p=0.000). The majority of these mutations were GC → TA transversion mutations (60%). However, patients with OC presented mutations in all the exons and introns studied. Highest diagnostic accuracy (p=0.0001) was observed when incorporating alcohol and tobacco habits variables with TP53 mutations. Conclusions: Our results prove to be statistically reliable, with parameter estimates that are nearly unbiased even for small sample sizes. Models 2 and 3 were the most accurate for assessing the risk of an OPMD becoming cancerous. However, in a public health context, model 3 is the most recommended because the characteristics considered are easier and less costly to evaluate.; Objetivos: El objetivo de este trabajo fue evaluar los hábitos de riesgo, los fenotipos clínicos y celulares y los cambios en el ADN de TP53 en muestras de mucosa oral de pacientes con Trastornos Orales Potencialmente Malignos (OPMD), para crear modelos que permitan obtener patrones genotípicos y fenotípicos que determinen el riesgo de que las lesiones se vuelvan malignas. Diseño del estudio: En las historias clínicas se recogieron fenotipos clínicos, antecedentes familiares de cáncer y hábitos de riesgo. Se estudió la mutación del gen TP53 y las características morfométricas-morfológicas, y se aplicaron modelos multivariantes. Se establecieron tres grupos: a) grupo de cáncer oral (OC) (n=10), b) grupo de OPMD (n=10), y c) grupo control (n=8). Resultados: Se observó que una media del 50% de los pacientes con neoplasia tenían hábitos de fumar y beber. Se observó un alto porcentaje de mutaciones TP53 en las lesiones de OC (30%) y OPMD (media del 20%) (p=0,000). La mayoría de estas mutaciones eran mutaciones de transversión GC → TA (60%). Sin embargo, los pacientes con OC presentaron mutaciones en todos los exones e intrones estudiados. La mayor precisión diagnóstica (p=0,0001) se observó al incorporar las variables de hábitos de alcohol y tabaco con las mutaciones de TP53. Conclusiones: Nuestros resultados demuestran ser estadísticamente fiables, con estimaciones de parámetros casi insesgadas incluso para tamaños de muestra pequeños. Los modelos 2 y 3 fueron los más precisos para evaluar el riesgo de que un OPMD se vuelva canceroso. Sin embargo, en un contexto de salud pública, el modelo 3 es el más recomendable porque las características consideradas son más fáciles y menos costosas de evaluar.
2013-01-01T00:00:00ZIn vitro evaluation of the film thinkness of self-adhesive resin cementsChávez Lozada, JulioUrquía Morales, María del Carmenhttp://hdl.handle.net/11086/134892024-02-09T13:23:25Z2013-01-01T00:00:00ZIn vitro evaluation of the film thinkness of self-adhesive resin cements
Chávez Lozada, Julio; Urquía Morales, María del Carmen
El objetivo fue evaluar el espesor de película de cementos resinosos de autograbado. Materiales y Métodos: se utilizaron los siguientes materiales Grupo 1: Relyx U100 (3M /ESPE), Grupo 2 BisCem (Bisco), Grupo 3: Max Cem (Kerr), Grupo 4: Set (SDI) y Grupo 5: Relyx ARC (3M/ESPE) como control. Se emplearon dos superficies de vidrio de 25.4 x 76.2 x 1 mm., que fueron señaladas en su parte media con una marca, para ubicar el material sobre la misma área. Se utilizó un volumen de 0,05 ml. de material para cada probeta. Se esperó un tiempo de 1, 3 o 6 minutos para aplicar una carga de 50 N durante 1 minuto. Trascurrido dicho lapso cada probeta se sometió a lectura de espesor de película utilizando un micrómetro digital, con una precisión de 1um; (Digimatic, Mitutoyo Corporation, Japón.). Resultados: Los datos fueron analizados mediante ANOVA y test de comparaciones múltiples de Bonferroni, no hubo diferencias significativas entre los materiales evaluados (p = 0,0921), ni en la interacción material / tiempo (p = 0,0864), pero si existieron diferencias en relación al factor tiempo (p = 0,0001). Al minuto el menor espesor de película correspondió a Relyx ARC (control) con un valor de 14 µm, seguido por Relyx U100 (17 µm), BisCem, Maxcem y SeT presentaron un valor de 19 µm. A los 3 minutos, el grupo 5 (control) presentó también el menor espesor (19 µm), seguido en orden creciente por el grupo 1 (21 µm), grupo 3 (25 µm), grupo 2 (29 µm) y grupo 4 (31 um). A los 6 minutos, el grupo 4 mostró el menor valor con 34 µm, seguido por grupo 1 (38 µm), grupo 5 (40 µm), grupo 2 (41 µm) y grupo 3 (42 µm). Conclusiones: El espesor de película de los cementos resinosos se vió influenciado por el tiempo y reacción de polimerización. Los cementos de autograbado presentaron un reducido espesor de película.; The aim of this study was to evaluate the film thickness of selfetching resin cement. The following materials were used:
Group1, Relyx U100 (3M /ESPE); Group 2, BisCem (Bisco);
Group 3, Max Cem (Kerr); Group 4, Set (SDI) and Group 5,
Relyx ARC (3M/ESPE) as control. Two 5.4 x 76.2 x 1 mm glass
slides were marked in the center to identify the area where
the material would be placed. A volume of 0.05 ml was used
for each specimen material. After 1, 3 and 6 minutes, a 50 N
load was applied for one minute. The thickness of each specimen was then measured using a digital micrometer to the
nearest 1 µm; (Digimatic, Mitutoyo Corporation, Japan.).
Data were analyzed using ANOVA and Bonferroni’s multiple
comparison tests. No significant difference was found between
the materials tested (p = 0.0921) or material/time interaction
(p = 0.0864), but there were differences in the time factor
(p = 0.0001). At one minute, the thinnest film was Relyx ARC
(control) (14 µm), followed by Relyx U100 (17 µm), and Maxcem and SeT (19 µm). At 3 minutes, Group 5 (control) was
also the thinnest film (19 µm), followed by Group 1 (21 µm),
Group 3 (25 µm), Group 2 (29 µm), and Group 4 (31 µm). At
6 minutes, Group 4 was the thinnest (34 µm), followed by
Group 1 (38 µm), Group 5 (40 µm), Group 2 (41 µm) and
Group 3 (42 µm). The film thickness of resin cements was
influenced by time and polymerization reaction. The film
thickness of self-etching cements was low.
2013-01-01T00:00:00Z