dc.contributor.author | Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. | |
dc.date.accessioned | 2019-10-29T16:05:41Z | |
dc.date.available | 2019-10-29T16:05:41Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | | |
dc.identifier.uri | http://dx.doi.org/10.3290/j.jad.a29588 | |
dc.identifier.uri | http://hdl.handle.net/11086/13456 | |
dc.description.abstract | Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used. Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts. | en |
dc.description.uri | | |
dc.format.medium | Impreso; Electrónico y/o Digital | |
dc.language.iso | eng | en |
dc.publisher | | |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS | en |
dc.title | An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements | en |
dc.type | article | en |
dc.description.version | publishedVersion | |
dc.journal.city | Londres | |
dc.journal.country | Reino Unido | |
dc.journal.editorial | QUINTESSENCE PUBLISHING CO INC | |
dc.journal.pagination | 541-546 | |
dc.journal.referato | Con referato | |
dc.journal.title | ISSN: 1461-5185 - THE JOURNAL OF ADHESIVE DENTISTRY. | |
dc.journal.tome | | |
dc.journal.volume | 6 | |
dc.description.field | Odontología, Medicina y Cirugía Oral | |
dc.identifier.eissn | | |